胶束体系中曲克芦丁的荧光特性及其分析应用

对曲克芦丁在胶束体系中的荧光性质进行了研究。实验发现在NaAc-HAc缓冲体系中，曲克芦丁自身具有较弱的内源性荧光，加入适量的表面活性剂十二烷基苯磺酸钠-6(SDBS-6)，可使曲克芦丁与SDBS-6形成胶束配合物，体系微环境发生改变，使荧光发射强度显著增强，荧光增强程度与曲克芦丁的含量在一定范围内呈良好的线性关系。据此提出了基于SDBS-6增敏胶束荧光光谱法测定微量曲克芦丁的新方法。对测定条件进行了详细研究，结果表明： 在pH 5.53的NaAc-HAc缓冲体系中，当最大激发波长λex=350.0 nm，最大发射波长λ_em=456.3 nm时，方法线性范围为1.6×10-6 mol·L-1～8.0×10-10 mol·L-1,检出限1.1×10-10 mol·L-1,相对标准偏差为1.7%(n=11,c=6.0×10-7 mol·L-1)。方法用于片剂和注射液中曲克芦丁的测定，回收率为98.5%～100.4%，结果令人满意。

The Fluorescence Characteristics of Troxerutin in Micellar System and Their Application

The fluorescence characteristics of the troxerutin in micellar system were studied. The experiments indicate that troxerutin can creat initial fluorescence in NaAc-HAc buffer solution and the existence of sodium dodecly benzen sulfonate-6(SDBS-6) can enhance the fluorescence signal of troxerutin greatly. A simple and highly sensitive fluorescence method using the micellar system for the trace analysis of troxerutin has been developed. The experimental conditions that influence the fluorescence intensity were investigated and optimized. The optimum experimental conditions are as follows: in pH 5.53 NaAc-HAc buffer solution,the excitation and emission wavelengths are 350.0 and 456.3 nm,respectively,and the concentration of SDBS-6 is 0.25%. The linear range is 1.6×10-6 mol·L-1-8.0×10-10 mol·L-1 with the detection limit of 1.1×10-10 mol·L-1. The RSD is 1.7%(n=11,c=6.0×10-7 mol·L-1),and the recovery is within the range of 98.5%-100.4%. The method has been successfully applied to the determination of troxerutin in tablets and injections. The results are satisfactory.