| 以Cu(II)为光谱探针离子分光光度法测定异烟肼的新方法 |
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| 引用本文： | 仉华 a,c,张炎b,李全民 a,杜新贞c. 以Cu(II)为光谱探针离子分光光度法测定异烟肼的新方法[J]. 中国化学, 2009, 27(3): 518-522. DOI: 10.1002/cjoc.200990085 |
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| 作者姓名： | 仉华 a c 张炎b 李全民 a 杜新贞c |
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| 作者单位： | a 河南师范大学化学与环境科学学院b河南科技学院化学化工学院 ; ;c西北师范大学化学化工学院 ; |
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| 摘 要： | 以Cu(II)为光谱探针建立了一种高选择性、高灵敏度测定异烟肼的新方法。试验表明：在pH 6.0时，Cu(II)可被异烟肼还原生成Cu(I)，反应生成的Cu(I) 与SCN-反应生成CuSCN白色乳状沉淀，在硝酸钠的存在下该沉淀可被浮选至水相表面。通过测定水相中剩余Cu(II)的量，可以间接测定异烟肼的含量。已反应Cu(II) 的量与异烟肼的浓度呈良好的线性关系，线性范围为0.050-4.50 µg mL-1，检出限为0.048 µg mL-1。该法已经成功的用于药物样品和病人尿液样品中异烟肼含量的测定。
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| 关 键 词： | Cu(II) 光谱探针离子 异烟肼 分光光度法 |
| 收稿时间： | 2008-05-24 |
| 修稿时间： | 2008-09-11 |
A Novel Spectrophotometric Method for the Determination of Isoniazid Using Cu(II) as Spectroscopic Probe Ion |
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| Hua ZHANG,Yan ZHANG,Xianjun WEI,Xinzhen DU,Quanmin LI. A Novel Spectrophotometric Method for the Determination of Isoniazid Using Cu(II) as Spectroscopic Probe Ion[J]. Chinese Journal of Chemistry, 2009, 27(3): 518-522. DOI: 10.1002/cjoc.200990085 |
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| Authors: | Hua ZHANG Yan ZHANG Xianjun WEI Xinzhen DU Quanmin LI |
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| Affiliation: | 1. College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, Henan 453007, China;2. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, China;3. College of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang, Henan 453003, China;4. Tel.: 0086‐0373‐3326336;5. Fax: 0086‐0373‐3326336 |
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| Abstract: | A novel method to determine isoniazid with high sensitivity and good selectivity has been established by using Cu(II) as a spectroscopic probe ion. The experiment indicated that Cu(II) was reduced to Cu(I) by isoniazid at pH 6.0, then the resulting Cu(I) was precipitated with SCN? to form CuSCN that could be stayed upon the surface of water phase in the presence of NaNO3. By determining the residual amount of Cu(II) in the water phase, the indirect determination of isoniazid could be achieved. Good linear relationship between the amount of the reacted Cu(II) and the concentration of isoniazid was observed, with the linear range being 0.050–4.50 µg·mL?1, and the detection limit being 0.048 µg·mL?1. This method has been successfully applied to determining isoniazid among pharmaceutical samples and patient urine samples. |
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| Keywords: | Cu(II) spectroscopic probe ion isoniazid spectrophotometry |
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