| 酸性品红共振光散射法测定蛋白质 |
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| 引用本文： | 张红医,刘保生,张红蕾,赵勇. 酸性品红共振光散射法测定蛋白质[J]. 光谱学与光谱分析, 2002, 22(6): 1054-1056 |
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| 作者姓名： | 张红医 刘保生 张红蕾 赵勇 |
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| 作者单位： | 河北大学化学与环境科学学院,河北,保定,071002 |
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| 摘 要： | 本文研究了酸性品红 (FA)与蛋白质相互作用所引起的共振光散射 (RLS)光谱 ,在pH 1 88～ 4 4 8范围内 ,加入蛋白质导致酸性品红 (FA)共振光散射增强 ,在 340nm处 ,存在一共振光散射增强峰 ,其散射光强度增加值与蛋白质的浓度呈线性关系 ,据此建立了一种测定蛋白质的共振散射法。方法的线性范围为 0～ 2 5mg·L- 1 ,检出限 0 0 2 3mg·L- 1 。该方法用于人尿样和血样的测定 ,与医院提供结果相符 ,且具有更高的灵敏度。
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| 关 键 词： | 测定 共振光散射 酸性品红 人血清白蛋白 牛血清白蛋白 临床分析 尿样 血样 |
| 文章编号： | 1000-0593(2002)06-1054-03 |
| 修稿时间： | 2001-08-24 |
Determination of Protein Concentration by the Enhancement of Rayleigh Light Scattering of Fuchsine Acid |
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| Hong-yi Zhang,Bao-sheng Liu,Hong-lei Zhang,Yong Zhao. Determination of Protein Concentration by the Enhancement of Rayleigh Light Scattering of Fuchsine Acid[J]. Spectroscopy and Spectral Analysis, 2002, 22(6): 1054-1056 |
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| Authors: | Hong-yi Zhang Bao-sheng Liu Hong-lei Zhang Yong Zhao |
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| Affiliation: | College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China. |
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| Abstract: | A new Rayleigh light scattering (RLS) assay is presented in this paper. At the optimum pH = 2.72, the weak RLS of fuchsine acid can be greatly enhanced by the addition of proteins due to the interaction between protein and fuchsine acid. A new quantitative determination method for proteins has been developed. The linear range for human serum albumin is 0-4.0 mg.L-1 with detection limit of 23 micrograms.L-1. Besides high sensitivity, the method is characterized by good reproducibility, rapidity of reaction, good stability and few interfering substances. The determination results of the proteins in human serum and urine samples are very close those obtained using Biuret method, with relative stand deviation of 0.94%-4.93%. |
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| Keywords: | Rayleigh light scattering Fuchsine acid Human serum albumin Bovine serum albumin |
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