Identification of epigenetic DNA modifications with a protein nanopore |
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| Authors: | Wallace Emma V B Stoddart David Heron Andrew J Mikhailova Ellina Maglia Giovanni Donohoe Timothy J Bayley Hagan |
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| Institution: | Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, UK OX1 3TA. |
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| Abstract: | Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA. |
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