Sandwich probes: two simultaneous reactions for templated nucleic acid detection |
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| Authors: | Kleinbaum Daniel J Kool Eric T |
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| Affiliation: | Department of Chemistry, Stanford University, Stanford, CA 94305, USA. |
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| Abstract: | Fluorescence-quenched nucleic acid probes with reactive moieties at both the 5' and 3' ends are synthesized and tested for reaction with two adjacent nucleophile-containing DNAs. These probes improve signal to background over singly reactive probes and can discriminate single nucleotide polymorphisms in the target DNA or RNA. |
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