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Three-dimensional thin-layer chromatography
Authors:V. G. Berezkin and N. Yu. Kulakova
Affiliation:(1) Research Center for Analytical Sciences, Northeastern University, Box 332, Shenyang, 110004, China;(2) Department of Cell Biology, Key Laboratory of Cell Biology for Ministry of Public Health, China Medical University, Shenyang, 110001, China;
Abstract:A three-dimensional variation of planar chromatography is proposed and realized. In this new variation of planar chromatography, components of the mixture under investigation migrate under the action of the mobile phase flow in one direction, then in another direction at an angle to the first direction, and then in a third direction at an angle to the second direction; in this case, all elution processes are carried out using different volatile eluents and drying the plate between the three elution processes. Using the example of separation of a mixture of dyes, it is shown that three-dimensional planar chromatography is appropriate for practical purposes.
Keywords:
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