Sequence-specific Hydrolysis of Single-stranded DNA by PNA-Cerium (Ⅳ) Adduct |
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作者姓名: | He Bai SHEN Feng WANG Yong Tao YANG |
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作者单位: | College of Life & Environment Science, Shanghai Normal University, Shanghai 200234 |
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摘 要: | A novel artificial site specific cleavage reagent, with peptide nucleic acid (PNA) as sequence-recognizing moiety and cerium (IV) ions as “scissors” for cleaving target DNA, was synthesized. Subsequently, it was employed in the cleavage of target 26-mer single-stranded DNA (ssDNA), which has 10-mer sequence complementary with PNA recognizer in the hybrids, under physiological conditions. Reversed-phase high-performance liquid chromatogram (RPHPLC) experiments indicated that the artificial site specific cleavage reagent could cleave the target DNA specifically.
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关 键 词: | 铈 缩氨酸 水解反应 染色体 DNA |
收稿时间: | 2005-03-28 |
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