Comparison of citric acid production by solid-state fermentation in flask, column, tray, and drum bioreactors |
| |
Authors: | Luciana P S Vandenberghe Carlos R Soccol F C Prado Ashok Pandey |
| |
Institution: | (1) Laboratório de Processos Biotecnológicos, Departamento de Engenharia Química, Universidade Federal do Paraná (UFPR), Caixa Postal 19011, 81531-970 Curitiba-PR, Brazil;(2) Biotechnology Division, Regional Research Laboratory, CSIR, 695 019 Trivandrum, India |
| |
Abstract: | Studies were conducted to evaluate citric acid production by solid-state fermentation (SSF) using cassava bagasse as substrate
employing a fungal culture of Aspergillus niger LPB 21 at laboratory and semipilot scale. Optimization of the process parameters temperature, pH, initial humidity, aeration,
and nutritive composition was conducted in flasks and column fermentors. The results showed that thermal treatment of cassava
bagasse enhanced fungal fermentation efficacy, resulting in 220 g of citric acid/kg of dry cassava bagasse with only treated
cassava bagasse as substrate. The results obtained from the factorial experimental design in a column bioreactor showed that
an aeration rate of 60 mL/min (3 mL/g·min]) and 60% initial humidity were optimum, resulting in 265.7 g/kg of dry cassava
bagasse citric acid production. This was almost 1.6 times higher than the quantities produced under unoptimized conditions
(167.4 g of citric acid/kg of dry cassava bagasse). The defined parameters were transferred to semipilot scale, which showed
high promise for large-scale citric acid production by SSF with cassava bagasse. Respirometry assays were carried out in order
to follow indirectly the biomass evolution of the process. Citric acid production reached 220, 309, 263, and 269 g/kg of dry
cassava bagasse in Erlenmeyer flasks, column fermentors, a tray bioreactor, and a horizontal drum bioreactor, respectively. |
| |
Keywords: | Citric acid solid-state fermentation respirometry column fermentors horizontal drum |
本文献已被 PubMed SpringerLink 等数据库收录! |
|