重金属铊胁迫羽衣甘蓝的in situ-ATR-FTIR表征

通过植物叶片光合作用消耗二氧化碳实验，实现了羽衣甘蓝光合作用的原位衰减全反射红外光谱表征。结果表明，羽衣甘蓝无论是否受到铊胁迫，其吸收峰的位置均无明显改变，其中3 380 cm-1处的强吸收峰表征叶片中的水分，碳水化合物，蛋白质和酰胺化合物等;2 916，2 850 cm-1处的强峰以及2 960 cm-1处的肩峰表征碳水化合物和脂肪族化合物等;1 640 cm-1处的吸收峰表征水分。然而，通过对羽衣甘蓝叶片进行原位表征，发现表征CO₂的2 360和2 340 cm-1双吸收峰(“负峰”)均从无到有，逐渐增强，说明羽衣甘蓝在进行光合作用。并且，铊胁迫组叶片二氧化碳的消耗速率明显高于空白样，即一定浓度铊胁迫会提高羽衣甘蓝的光合速率，表明羽衣甘蓝对重金属铊具有耐受的特性;结果也表明成年叶光合作用强度大于幼叶，这符合植物叶片光合作用的一般规律。

Characterization of Kale(Brassica Oberacea var Acephala) under Thallium Stress by In Situ Attenuated Total Reflection FTIR

The experiment was designed based on consumption of carbon dioxide through the photosynthesis of Brassica oberacea var acephala leaf,and the photosynthesis of kale leaf under thallium stress was investigated by in situ attenuated total reflection FTIR(in situ ATR-FTIR).The ATR-FTIR showed that the absorption peaks of leaves had no obvious difference between plants growing in thallium stress soil and plants growing in non-thallium pollution soil,and the strong peaks at 3 380 cm-1 could be assigned to the abs...