| 均匀设计在蚕蛹虫草和冬虫夏草活性成分分析中的应用 |
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| 引用本文： | 周枝凤,梁逸曾,胡芸,郭方遒.均匀设计在蚕蛹虫草和冬虫夏草活性成分分析中的应用[J].分析测试学报,2004,23(2):35-37,40. |
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| 作者姓名： | 周枝凤 梁逸曾 胡芸 郭方遒 |
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| 作者单位： | 中南大学,化学化工学院,中药现代研究中心,湖南,长沙,410083;中南大学,化学化工学院,中药现代研究中心,湖南,长沙,410083;中南大学,化学化工学院,中药现代研究中心,湖南,长沙,410083;中南大学,化学化工学院,中药现代研究中心,湖南,长沙,410083 |
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| 基金项目： | 国家自然科学基金重点项目 (20235020) |
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| 摘 要： | 采用均匀实验设计方法快速找到蚕蛹虫草活性成分的高效液相色谱最优分离条件；将该优化条件用于蚕蛹虫草和冬虫夏草的水溶性成分的分离取得满意结果，并对部分组分进行了定性定量分析；均匀实验设计方法可较快找到复杂未知或半未知体系的色谱分离条件。
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| 关 键 词： | 蚕蛹虫草 冬虫夏草 均匀设计 信息量 高效液相色谱法 |
| 文章编号： | 1004-4957(2004)02-0035-03 |
Uniform Design Applied to the Determination of Active Components of Cordyceps Militaris and Cordyceps Sinensis |
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| ZHOU Zhi-feng,LIANG Yi-zeng,HU Yun,GUO Fang-qiu.Uniform Design Applied to the Determination of Active Components of Cordyceps Militaris and Cordyceps Sinensis[J].Journal of Instrumental Analysis,2004,23(2):35-37,40. |
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| Authors: | ZHOU Zhi-feng LIANG Yi-zeng HU Yun GUO Fang-qiu |
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| Abstract: | The optimal HPLC chromatographic conditions for the separation of active components in Cordyceps militaris were found with the aid of uniform design. As the optimized conditions were applied to separate the water-soluble active constituentes in extracts from Cordyceps militaris and Cordyceps sinensis, satisfactory results were obtained. Both qualitative and quantitative analyses of some active constituents have been conducted on the basis of optimized separation. Hence, uniform design is a good method to optimize the chromatographic separation conditions of complicated systems with unknown or uncertain components. |
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| Keywords: | Cordyceps militaris Cordyceps sinensis Uniform design Information quantity HPLC |
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