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Determination of apparent reducing sugars, moisture and acidity in honey by attenuated total reflectance-Fourier transform infrared spectrometry
Authors:Luiz C.M. Pataca   Waldomiro Borges Neto   Maria C. Marcucci  Ronei J. Poppi  
Affiliation:

aInstituto de Química, UNICAMP, P.O. Box 6154, 13084-971 Campinas, SP, Brazil

bUniversidade Bandeirante de São Paulo, Campus MC, Rua Maria Cândida, 1813, 02071-013 São Paulo, SP, Brazil

Abstract:Attenuated total reflectance-Fourier transform infrared spectrometry, in conjunction with multivariate calibration, was used for determination of reducing sugars, humidity and acidity in honey bee samples. Multivariate calibration models were built using partial least squares (PLS) and were refined through variable selection per interval (iPLS) and genetic algorithms. The calibration models show satisfactory results for all parameters with average relative errors of 6% for acidity, 1% for reducing sugars and 2% for humidity. For the acidity and reducing sugars parameters, variable selection was irrelevant, but for humidity it was essential. For the humidity parameter, it was necessary to use two variable selection techniques (by intervals and genetic algorithm) concomitantly in order to obtain a satisfactory calibration model.
Keywords:Honey   Infrared spectroscopy   ATR   PLS
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