The Methane Monooxygenase Intrinsic Activity of Kinds of Methanotrophs |
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Authors: | Yingxin Zhang Jiaying Xin Linlin Chen Chungu Xia |
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Institution: | (1) State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, People’s Republic of China;(2) College of Food Engineering, Harbin University of Commerce, Harbin, 150076, People’s Republic of China;(3) Graduate University of Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China |
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Abstract: | Methanotrophs have promising applications in the epoxidation of some alkenes and some chlorinated hydrocarbons and in the
production of a biopolymer, poly-β-hydroxybutyrate (poly-3-hydroxybutyrate; PHB). In contrast with methane monooxygenase (MMO)
activity and ability of PHB synthesis of four kinds of methanotrophic bacteria Methylosinus trichosporium OB3b, M. trichosporium IMV3011, Methylococcus capsulatus HD6T, Methylomonas sp. GYJ3, and the mixture of the four kinds of strains, M. trichosporium OB3b is the highest of the four in the activity of propene epoxidation (10.72 nmol/min mg dry weight of cell dwc]), the
activity of naphthalene oxidation (22.7 mmol/mg dwc), and ability in synthesis of PHB(11% PHB content in per gram dry weight
of cell in 84 h). It could be feasible to improve the MMO activity by mixing four kinds of methanotrophs. The MMO activity
dramatically decreased when the cellular PHB accumulated in the second stage. The reason for this may be the dilution of the
MMO system in the cells with increasing PHB contents. It has been found that the PHB contents at the level of 1–5% are beneficial
to the cells for maintenance of MMO epoxidation activity when enough PHB have been accumulated. Moreover, it was also found
that high particulate methane monooxygenase activity may contribute to the synthesis of PHB in the cell, which could be used
to improve the yield of PHB in methanotrophs. |
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Keywords: | Methanotroph Methane monooxygenase Poly-β -hydroxybutyrate Reducing equivalent |
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