Fully automated screening of veterinary drugs in milk by turbulent flow chromatography and tandem mass spectrometry |
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Authors: | Alida A M Stolker Ruud J B Peters Richard Zuiderent Joseph M DiBussolo Cláudia P B Martins |
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Institution: | (1) RIKILT Institute of Food Safety, P.O. Box 230, 6700 AE Wageningen, The Netherlands;(2) Thermo Fisher Scientific, Takkebijsters 1, 4817, BL Breda, The Netherlands;(3) Chemistry Department, Thermo Fisher Scientific’s Applications Laboratory at West Chester University of Pennsylvania, 750 S. Church Street, West Chester, PA 19383, USA;(4) Thermo Fisher Scientific, Acero 30-32, Planta. 2, Mod. 3, 08038 Barcelona, Spain;; |
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Abstract: | There is an increasing interest in screening methods for quick and sensitive analysis of various classes of veterinary drugs
with limited sample pre-treatment. Turbulent flow chromatography in combination with tandem mass spectrometry has been applied
for the first time as an efficient screening method in routine analysis of milk samples. Eight veterinary drugs, belonging
to seven different classes were selected for this study. After developing and optimising the method, parameters such as linearity,
repeatability, matrix effects and carry-over were studied. The screening method was then tested in the routine analysis of
12 raw milk samples. Even without internal standards, the linearity of the method was found to be good in the concentration
range of 50 to 500 μg/L. Regarding repeatability, RSDs below 12% were obtained for all analytes, with only a few exceptions.
The limits of detection were between 0.1 and 5.2 μg/L, far below the maximum residue levels for milk set by the EU regulations.
While matrix effects—ion suppression or enhancement—are obtained for all the analytes the method has proved to be useful for
screening purposes because of its sensitivity, linearity and repeatability. Furthermore, when performing the routine analysis
of the raw milk samples, no false positive or negative results were obtained. |
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