| CdSe/ZnS量子点探针用于检测猪链球菌2型溶菌酶释放蛋白(MRP)抗原的新方法研究 |
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| 引用本文： | 武红敏,韩鹤友,金梅林,张安定. CdSe/ZnS量子点探针用于检测猪链球菌2型溶菌酶释放蛋白(MRP)抗原的新方法研究[J]. 化学学报, 2009, 67(10): 1087-1092 |
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| 作者姓名： | 武红敏 韩鹤友 金梅林 张安定 |
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| 作者单位： | 华中农业大学理学院农业微生物学国家重点实验室,武汉,430070;华中农业大学动物科技学院,农业微生物学国家重点实验室,武汉,430070 |
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| 基金项目： | 国家自然科学基金，湖北省自然科学基金重点项H，武汉市学科带头人计划，国家973计划 |
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| 摘 要： | 合成了高发光效率的CdSe/ZnS量子点并制备了CdSe/ZnS量子点-溶菌酶释放蛋白(MRP)抗体探针, 利用凝胶电泳和分子光谱法研究了MRP抗体与CdSe/ZnS量子点的结合机理. 荧光光谱法优化了CdSe/ZnS量子点-MRP抗体探针制备的影响因素, 建立了一种测定MRP抗原的新方法, 其线性范围为5.0×10－8～1.5×10－6 mol/L, 线性相关系数为0.9976, 检测限为 1.9×10－8 mol/L.
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| 关 键 词： | 猪链球菌2型 溶菌酶释放蛋白(MRP) CdSe/ZnS量子点探针 检测 |
| 收稿时间： | 2008-07-04 |
| 修稿时间： | 2008-12-20 |
A Novel Method to Detect Muramidase Released Protein(MRP) Antigen of Streptococcus sui Serotype 2 Based on CdSe/ZnS Quantum Dot Probe |
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| Wu Hongmin,Han Heyou,Jin Meilin,Zhang Anding. A Novel Method to Detect Muramidase Released Protein(MRP) Antigen of Streptococcus sui Serotype 2 Based on CdSe/ZnS Quantum Dot Probe[J]. Acta Chimica Sinica, 2009, 67(10): 1087-1092 |
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| Authors: | Wu Hongmin Han Heyou Jin Meilin Zhang Anding |
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| Affiliation: | (a College of Science, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070) (b College of Veterinary Medicine, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070) |
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| Abstract: | High-quality CdSe/ZnS quantum dot and the CdSe/ZnS quantum dot-muramidase released protein (MRP) antibody probe were prepared, and the conjugation mechanism was studied according to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and molecular spectrome- try. The factors influencing the preparation of CdSe/ZnS quantum dot-MRP antibody probe were optimized by fluorescence spectrometry. Under the optimum conditions, the probe showed good sensitivity to MRP antigen. The cali... |
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| Keywords: | Streptococcus Suis serotype 2 muramidase released protein (MRP) CdSe/ZnS quantum dot probe detection |
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