Determination of chloramphenicol in royal jelly by liquid chromatography/tandem mass spectrometry |
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Authors: | Jiang Yuan Xu Jin-Zhong Shen Chong-Yu Ding Tao Chen Hui-Lan Wu Bin Shen Wei-Jian |
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Institution: | Jiangsu Entry-Exit Inspection and Quarantine Bureau, APFIC, Food Laboratory, 99 Zhonghua Rd, Nanjing, People's Republic of China. |
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Abstract: | A liquid chromatographic/tandem mass spectrometric method was developed and validated for the determination of chloramphenicol (CAP) in royal jelly. Royal jelly samples were first denatured with lead acetate solution, and the CAP was extracted with solid-phase extraction before separation by liquid chromatography. A triple-quadrupole mass spectrometer operated in the negative electrospray ionization and selected-reaction monitoring mode was used for the detection of CAP. For method validation, royal jelly samples were fortified at CAP levels between 0.1 and 10.0 microg/kg; at these levels, recovery values (internal standard-corrected) ranged from 93.3 to 105.0%, and the within-laboratory reproducibility (relative standard deviation) was < or = 9.1%. The decision limit was 0.07 microg/kg, and the detection capability was 0.1 microg/kg. |
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