Characterisation of 3-methylorcinaldehyde synthase (MOS) in Acremonium strictum: first observation of a reductive release mechanism during polyketide biosynthesis |
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| Authors: | Bailey Andrew M Cox Russell J Harley Kate Lazarus Colin M Simpson Thomas J Skellam Elizabeth |
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| Affiliation: | School of Biological Sciences, University of Bristol, Woodland Road, Bristol, UK BS8 1UG. |
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| Abstract: | Isolation and sequencing of a PKS gene isolated from xenovulene-producing cultures of Acremonium strictum indicated the presence of NT-, KS-, AT-, PT-, C-MeT- and R-domains; heterologous expression in Aspergillus oryzae resulted in the production of 3-methylorcinaldehyde, demonstrating the role of the terminal reductase domain in product release. |
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