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A radioimmunoassay for serum estradiol
Authors:Aruna Korde  Meera Venkatesh  S A Balakrishnan  Ramji Lal
Institution:(1) Isotope Division, Bhabha Atomic Research Centre, 400085 Bombay, India;(2) Radiopharmaceuticals Operations, BRIT, 400094 Bombay, India
Abstract:A radioimmunoassay procedure to measure estradiol levels in human serum was developed using125I labeled estradiol and antiestradiol antibody raised in-house using the estradiol-6-CMO-BSA as the immunogen. Controlled process serum was used to maintain the serum matrix and sample-standard identity. The assay was validated by analysing several (n=40) samples and comparing with established commercial assays. The assay had a sensitivity of 35 pg/mL and a range of 50 pg/mL to 5 ng/mL. The interassay C.V. were between 2 to 10% while the intraassay C.V. were between 2 and 8%. This assay is sensitive enough for monitoring ovulation and hypersecretions in ovarian tumours and was primarily developed to be used as an in-house assay in the local hospitals.
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