Novel quantitative electrophoretic analysis of endotoxins on microchips |
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Authors: | Kilár Anikó Farkas Viktor Kovács Krisztina Kocsis Béla Kilár Ferenc |
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Affiliation: | Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Pécs, Hungary. |
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Abstract: | A novel microchip electrophoresis method was developed and applied for sensitive detection and quantitative analysis of endotoxins extracted from Gram-negative bacteria. The method provides a fast and quantitative differentiation of smooth and rough endotoxins based on the solubilization and complexation of the lipopolysaccharides with dodecylsulfate, and then with a fluorescent dye. The migration of the complexes was followed by LIF detection. The novel method is able to replace the SDS-PAGE with the advantage of high speed and better sensitivity, and by avoiding the laborious gel-preparation and silver staining. |
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