首页 | 本学科首页   官方微博 | 高级检索  
     


AGR2, a mucinous ovarian cancer marker, promotes cell proliferation and migration
Authors:Park Kyoungsook  Chung Yong Jin  So Hyekyung  Kim Kwangsoo  Park Junsoo  Oh Mijoung  Jo Minwha  Choi Kyusam  Lee Eun-Ju  Choi Yoon-La  Song Sang Yong  Bae Duk-Soo  Kim Byoung-Gie  Lee Je-Ho
Affiliation:Samsung Biomedical Research Institute, Seoul, Korea.
Abstract:Ovarian cancer is a leading cause of death in women. Early detection of ovarian cancer is essential to decrease mortality. However, the early diagnosis of ovarian cancer is difficult due to a lack of clinical symptoms and suitable molecular diagnostic markers. Thus, identification of meaningful tumor biomarkers with potential clinical application is clearly needed. To search for a biomarker for the early detection of ovarian cancer, we identified human anterior gradient 2 (AGR2) from our systematic analysis of paired normal and ovarian tumor tissue cDNA microarray. We noted a marked overexpression of AGR2 mRNA and protein in early stage mucinous ovarian tumors compared to normal ovarian tissues and serous type ovarian tumors by Western blot analysis and immunohistochemistry. To further elucidate the role of AGR2 in ovarian tumorigenesis, stable 2774 human ovarian cancer cell lines overexpressing AGR2 were established. Forced expression of AGR2 in 2774 cells enhanced the growth and migration of ovarian cancer cells. AGR2 protein was detected in the serum of mucinous ovarian cancer patients by Western blot and ELISA analysis. Thus, AGR2 is a potential biomarker for the diagnosis of mucinous ovarian cancer and an ELISA assay may facilitate the early detection of mucinous ovarian cancer using patient serum.
Keywords:AGR2 protein, human   biological markers   ovarian neoplasms
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号