In vitro antimicrobial activity of extracts and compounds isolated from Cladonia uncialis |
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| Authors: | El?bieta Studzińska-Sroka El?bieta Ho?derna-K?dzia Agnieszka Galanty Wies?awa Bylka Karol Kacprzak Karolina ?wiklińska |
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| Institution: | 1. Department of Pharmacognosy, Poznan University of Medical Sciences, ?wi?cickiego 4, 60-781 Poznań, Polandela_studzinska@op.pl;3. Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznań, Poland;4. Department of Pharmacognosy, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, Poland;5. Department of Pharmacognosy, Poznan University of Medical Sciences, ?wi?cickiego 4, 60-781 Poznań, Poland;6. Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland;7. Department of Laboratory Diagnostics, Josef Strus Multidisciplinary Municipal Hospital, Szwajcarska 3, 61-285 Poznań, Poland |
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| Abstract: | Heptane (Hep), diethyl ether (Et2O), acetone (Me2CO) and methanolic (MeOH) extracts, as well as ( ? )-usnic acid and squamatic acid, were obtained from thallus of Cladonia uncialis (Cladoniaceae). The antimicrobial activities of these extracts, ( ? )-usnic acid and squamatic acid, were tested against reference strains: Staphylococcus aureus, Escherichia coli and Candida albicans. In addition, Me2CO extract was analysed against 10 strains of Methicillin-resistant S. aureus (MRSA) isolated from patients. All extracts exerted antibacterial activity against the reference strain S. aureus, comparably to chloramphenicol minimum inhibitory concentration (MIC) = 5.0 μg/mL]. The Me2CO extract exhibited the strongest activity against S. aureus (MIC = 0.5 μg/mL), higher than ( ? )-usnic acid, whereas squamatic acid proved inactive. The Me2CO extract showed potent antimicrobial activity against MRSA (MIC 2.5–7.5 μg/mL). Also no activity of C. uncialis extracts against E. coli and C. albicans was observed. |
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| Keywords: | Cladonia uncialis extracts usnic acid squamatic acid antimicrobial activity |
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