Fluorescence Study of Lipid-based DNA Carriers Properties: Influence of Cationic Lipid Chemical Structure |
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Authors: | Laure Burel-Deschamps Mathieu Mével Damien Loizeau Farouk Ayadi Jean-Jacques Yaouanc Jean-Claude Clément Paul-Alain Jaffrès Philippe Giamarchi |
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Institution: | (1) CEMCA, UMR CNRS 6521 Chemistry Department, University of Brest, 6 Avenue Le Gorgeu, 29238 Brest, France |
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Abstract: | We report here a study on the physicochemical properties of cationic phospholipids liposomes used for lipoplex formulation
and DNA transfer. The original cationic phospholipids synthesized in our laboratory are first presented with the liposome
formulation process. The second part deals with the liposomes fusogenic properties studied by fluorescence resonant energy
transfer (FRET). The nature of the cationic polar head and the formulation with or without a neutral colipid have a great
influence on the FRET signal. The third part reports the study of the viscosity of the liposome by fluorescence anisotropy
measurements. It has been observed that the vectors having a saturated lipid chain exhibit a more pronounced anisotropy than
those having unsaturated lipid chains. Finally, liposomes formed by a mixture of phospholipids and DC-Chol (a rigid lipid)
leads to increase the anisotropy denoting a more rigid liposome. |
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Keywords: | Fluorescence FRET Anisotropy Cationic phospholipids Liposomes |
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