Elecampane (Inula helenium) Root Extract and Its Major Sesquiterpene Lactone,Alantolactone, Inhibit Adipogenesis of 3T3-L1 Preadipocytes |
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| Authors: | Yeon-Seop Jung Yun-Jeong Jeong Joung-Hee Kim Chang-Hwan Jeon Syng-Ook Lee |
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| Affiliation: | 1.Department of Food Science and Technology, Keimyung University, Daegu 42601, Korea; (Y.-S.J.); (C.-H.J.);2.Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Korea;3.Department of Medicine, Research Institute of Biomedical Engineering, Catholic University of Daegu School of Medicine, Daegu 42472, Korea;4.Laboratory of Molecular Toxicology, College of Pharmacy, Pusan National University, Pusan 46241, Korea; |
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| Abstract: | Recent studies have shown that Nur77 and AMPKα play an important role in regulating adipogenesis and isoalantolactone (ISO) dual-targeting AMPKα and Nur77 inhibits adipogenesis. In this study, we hypothesized that Inula helenium (elecampane) root extract (IHE), which contains two sesquiterpene lactones, alantolactone (ALA) and ISO, as major compounds, might inhibit adipogenesis. Here, we found that ALA and IHE simultaneously target AMPKα and Nur77 and inhibited adipogenic differentiation of 3T3-L1 cells, accompanied by the decreased expression of adipocyte markers. Further mechanistic studies demonstrated that IHE shares similar mechanisms of action with ISO that reduce mitotic clonal expansion during the early phase of adipogenic differentiation and decrease expression of cell cycle regulators. These results suggest that IHE inhibits adipogenesis, in part, through co-regulation of AMPKα and Nur77, and has potential as a therapeutic option for obesity and related metabolic dysfunction. |
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| Keywords: | adipogenesis, Nur77, AMPKα , alantolactone, Inula helenium, elecampane |
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