Spektralphotometrische Bestimmung von Val5-Angiotensin II Asp1-β-amid durch Azokupplung mit p-Chlorbenzoldiazoniumchlorid |
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Authors: | W Vogt Kh R Koczorek H -H Vogt M Knedel Gerda Rindfleisch |
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Institution: | 1. 1. Medizinische Klinik der Universit?t München, Bundesrepublik Deutschland
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Abstract: | Through the azocoupling of Val5-angiotensin II-Asp1-β-amide (Hypertensin-CIBA) with p-halogene substituted benzenediazonium salts the possibility is shown for quantitative determination of polypeptides containing tyrosine and/or histidine. 1 to 2 nanomoles of the coupling-product could be measured photometrically. The detection limit can be lowered, if the polypeptide is coupled with a radioactive labelled diazonium salt. The preferable reaction conditions for diazotising and coupling are reported. The reaction-product showed an uniform behaviour in the used paper-chromatographic system. Probably it is a monoazo compound. Under the chosen conditions the tyrosyl group is very likely the reacting part of the hypertensin molecule. |
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