Fluorescence analysis of p-hydroxymethamphetamine in urine by thin-layer chromatography. |
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| Authors: | Noriyuki Kato Hiroaki Kubo Hiroshi Homma |
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| Affiliation: | Scientific Criminal Investigation Laboratory, Kanagawa Prefectural Police Headquarters, Yokohama, Japan. n-katoh@gd5.so-net.ne.jp |
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| Abstract: | A highly-sensitive analytical method for the detection of p-hydroxymethamphetamine (pOHMA) in urine is presented. The proposed method combines liquid-liquid extraction with acetonitrile and solid-phase extraction by thin-layer chromatography (TLC) with oxidation using potassium hexacyanoferrate(III) and sodium hydroxide to detect the fluorophor of pOHMA. The detection limit for pOHMA is 10 ng (n = 3). The analysis of pOHMA in forensic samples is successfully performed, without interference from endogenous fluorophors, yielding concentrations in the appropriate range for methamphetamine abusers. |
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