Single-stranded RNA as primers of terminal deoxynucleotidyl transferase for template-independent DNA polymerization |
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| Affiliation: | 1. School of Life Sciences, Tianjin University and Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin), Tianjin 300072, China;2. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China;3. Tianjin Key Laboratory of Pathogenic Microbiology of Infectious Disease, Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China;4. Department of Toxicology, Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China |
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| Abstract: | Terminal deoxynucleotidyl transferase (TdT) has been characterized as template-independent polymerase using single-stranded DNA (ssDNA) as primers to generate random oligonucleotides. However, the extension performance of TdT to single-stranded RNA (ssRNA) is vague. By systematically comparing and contrasting the performance of TdT-catalyzed ssDNA and ssRNA extension, it is indicated that the catalytic efficiency of ssRNA as primers was about 3 times lower than ssDNA as primers. Collectively, it is believed that understanding the catalytic performance of TdT will help to design the strategy to synthesize chimeric DNA on 3′-OH of ssRNA, which becomes invaluable. |
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