Ac36deoGlcNAz could selectively label O-GlcNAc modified proteins with minimal S-glyco-modification

A novel metabolic chemical reporter of Ac₃6deoGlcNAz was developed and confirmed as an effective probe for O-GlcNAc modification. Ac₃6deoGlcNAz labeling predominantly occurs in intracellular O-GlcNAcylated proteins rather than cell-surface glycoproteins. Of note, it could reduce the artificial S-glyco-modification compared to Ac₄GalNAz and Ac₄GlcNAz. This new reporter allows to be widely used in the field of proteomic identification of O-GlcNAcylation.