Determination of 9-[(2-phosphonylmethoxy)ethyl]adenine in rat urine by high-performance liquid chromatography with fluorescence detection. |
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| Authors: | J W Russell D Marrero V J Whiterock L J Klunk J E Starrett |
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| Institution: | Department of Metabolism and Pharmacokinetics, Bristol-Myers Squibb Company, Wallingford, CT 06492. |
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| Abstract: | A high-performance liquid chromatographic (HPLC) method for the determination of 9-(2-phosphonylmethoxy)ethyl]adenine (PMEA) in urine is described. The procedure includes treatment of the urine sample with chloroacetaldehyde to form the fluorescent 1,N6-ethenoadenosine derivative, which was analyzed by reversed-phase HPLC with fluorometric detection. Validation of the method showed good sensitivity, precision and reproducibility. The method is useful for the study of urinary excretion of PMEA in the rat. |
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