Determination of vitamins A, D, E, and K in human and bovine serum, and β-carotene and vitamin A palmitate in cosmetic and pharmaceutical products, by isocratic HPLC |
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Authors: | Lai-Hao Wang Shin-Hung Huang |
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Institution: | (1) Department of Applied Chemistry, China Nan University of Pharmacy and Science, Tainan, Taiwan 71710, ROC |
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Abstract: | Summary An HPLC method has been developed for the determination of fat-soluble vitamins. Ten fat-soluble vitamins were separated simultaneously
on a 25 cm×4.6 mm i.d. Hypersil C18 column with acetonitrile-dichloromethane-methanol, 60:20:20 (v/v) as mobile phase at 1.0 mL min−1 with wavelength-programmed ultraviolet-visible-absorbance detection. Total analysis time was 12 min. The limits of detection
were 0.03, 0.01, 0.55, 1.84, 0.02, 0.02, 0.01, 0.16, 0.33, 0.01, and 0.01 ng mL−1 for retinol, retinyl acetate, retinyl palmitate, β-carotene, ergocalciferol, cholecalciferol, tocopherol, tocopherol acetate,
phylloquinone, menatetrenone, and menadione, respectively. Analysis of human serum 2–7 days after ingestion of oral vitamins
and Chinese herbs led to the conclusion that the concentration of vitamins was higher than for control serum. |
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Keywords: | Column liquid chromatography Fat-soluble vitamins Human and bovine serum Cosmetic and pharmaceutical products |
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