| 微波消解衍生化气相色谱-质谱法测定鲨鱼软骨中的脂肪酸 |
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| 引用本文： | 李攻科,何小青,张展霞. 微波消解衍生化气相色谱-质谱法测定鲨鱼软骨中的脂肪酸[J]. 色谱, 2000, 18(4): 337-339 |
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| 作者姓名： | 李攻科 何小青 张展霞 |
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| 作者单位： | 中山大学化学系,广东,广州,510275 |
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| 基金项目： | 广东省自然科学基金博士启动基金! ( 9740 78)，教育部留学回国人员科研启动基金! ( 4 1 540 9)资助项目 |
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| 摘 要： | 研究了用微波技术消解衍生化气相色谱 质谱法 (GC MS)快速测定鲨鱼软骨中脂肪酸的分析方法。用正交设计试验优化消解衍生化条件 ,以盐酸 甲醇 (体积比为 1∶4)体系作消解衍生化溶液 ,在 60 0W微波功率作用下加热 4min进行样品的前处理 ,将样品的消解、脂肪酸的衍生化及脂肪酸甲酯的萃取融于一体。方法具有灵敏度高、省时、省试剂、操作方便等特点。该法适用于大批量固体样品中脂肪酸的测定。
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| 关 键 词： | 微波消解衍生化 气相色谱质谱法 鲨鱼软骨 脂肪酸 |
Determination of Fatty Acids in Shark Cartilage by GC-MS Using Microwave-Assisted Digestion and Derivatization |
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| LI Gong-ke,HE Xiao-qing,ZHANG Zhan-xia. Determination of Fatty Acids in Shark Cartilage by GC-MS Using Microwave-Assisted Digestion and Derivatization[J]. Chinese journal of chromatography, 2000, 18(4): 337-339 |
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| Authors: | LI Gong-ke HE Xiao-qing ZHANG Zhan-xia |
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| Affiliation: | Department of Chemistry, Zhongshan University, Guangzhou 510275, China. |
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| Abstract: | A rapid microwave-assisted digestion and derivatization method for the determination of fatty acids in shark cartilage by GC-MS was developed. The optimum conditions for digestion and derivatization were studied in detail using orthogonal design. The digestion and derivatization were accomplished in 4 minutes at 600 W microwave power using HCl-methanol (1:4, V/V) as digestion and derivatization solvent, and the extraction of the target analytes could be carried out simultaneously. This method is rapid, solvent-saving, and particularly suitable for the rapid determination of fatty acids in solid samples. |
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| Keywords: | microwave assisted digestion and derivatization gas chromatography mass spectrometry shark cartilage fatty acid |
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