液相色谱/电喷雾质谱测定柑桔中的柠檬苦素类似物配糖体

 利用液相色谱 /电喷雾质谱联用技术对柚皮中的柠檬苦素类似物配糖体进行了定向分析 ,在柚皮的乙醇提取物中检测出奥巴叩酮配糖体和诺米林配糖体。利用制备液相色谱对这两种柠檬苦素类似物配糖体进行纯化 ,并用核磁共振对其结构进行鉴定。测定结果表明 ,液质联用法不需标样即可对柑桔中的柠檬苦素类似物配糖体进行定性分析。方法准确、快速、简便。

Screening for Limonoid Glucosides in Citrus grandis L. Osbeck by High Performance Liquid Chromatography-Electrospray Ionization/Mass Spectrometry

A screening method for limonoid glucosides in the peel of Citrus grandis L Osbeck, utilizing high performance liquid chromatography (HPLC) with diode array detection (DAD), interfaced to electrospray ionization mass spectrometry (ESI MS), has been developed The methanol extract of citrus peel was preseparated with conventional polymer adsorption column and weak base anion ion exchange column and then the eluate from the column was analysed by LC ESI/MS In this paper, the UV Vis spectra and the mass spectra provide structural information without the necessity of isolating the individual compounds Two major limonoid glucosides obacunone glucoside (OG) and nomilin glucoside (NG) were identified in the methanol extract of the peel of Citrus grandis L Osbeck. Then the two limonoid glucosides were taken as the target and isolated by means of preparative HPLC on a C\-\{18\} reversed phase column with an acidic acetonitrile water mobile phase The structures of OG and NG were further confirmed by NMR spectrometry The results show that this method is rapid, convenient, sensitive and very useful for screening bioactive natural products