纳米雄黄与脂质体仿生膜的相互作用研究

本工作以卵磷脂与胆固醇组成的磷脂小单层脂质体（small unilamelarvesicles，suv）作为仿生膜的简单模型，采用表面等离子共振技术（SPR）、荧光偏振、拉曼（Raman）光谱、核磁共振（NMR）及原子力显微镜（AFM）研究纳米雄黄与SUV仿生膜的相互作用，证实了磷脂是纳米雄黄作用的关键靶分子．随纳米雄黄结合，SUV仿生膜的相对粘度聃值增大，膜的流动性减小．Raman光谱数据计算表明，作用后膜的纵向有序性参数s。。及横向有序性参数Slat值增大，说明纳米雄黄的结合使磷脂膜的脂酰基链全反式构型比例上升，膜的流动性减小．由Raman光谱和引PNMR结果推测，磷脂极性头部是纳米雄黄与磷脂的主要结合位点。AFM实时观测，纳米雄黄通过在膜表面打“孔”或“洞”的方式，损坏磷脂膜．

Studies on the biomimetic membrane interaction between liposome and realgar nanoparticles

The liposome of small unilamellar vesicles （SUV） made from phosphatidylcholine-cholesterol mixtures was used as a simple model for biomimetic membranes. The studies on the interaction between the liposome and realgar nanoparticles （NPs） demonstrate that the phospholipid is one of the key targeted molecules of realgar NPs, used by surface plasmon resonance （SPR） technology, fluorescence polarization, Raman spectroscopy, nuclear magnetic resonance （NMR） and atom force microscope （AFM）. It was observed that the relative viscosity （t/r） of the membrane increased and the membrane fluidity decreased as realgar NPs bound to SUV. Calculations of Raman intensity ratios detected the increase of longitudinal order parameters （Strans） and lateral order parameters （Slat） of the lipid bilayer, indicating a rise in the proportion of trans conformations of alkyl chains, and the decrease of membrane＇s fluidity attributed to the interaction of realgar NPs. Results of Raman spectra and 31p NMR suggest that the polar headgroup of phospholipid is the interacted target site of realgar NPs. Moreover, time-lapse AFM images show that realgar NPs compromised the phospholipid membrane integrity to result in the formation of “pore” or “hole”.