Determination of green-tea catechins in cosmetic formulations and in in-vitro skin extracts by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry |
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Authors: | M Frauen T Rode C Rapp H Steinhart |
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Institution: | (1) Institute of Biochemistry and Food Chemistry, Department of Food Chemistry, University of Hamburg, Grindelalle 117, 20146 Hamburg, Germany;(2) Institute of Pharmacy, Department of Pharmaceutical Technology and Biopharmacy, Christian-Albrechts-University, Gutenbergstrasse 76, 24118 Kiel, Germany;(3) Department of Analytical Research, Beiersdorf AG, Fkt. 4231, Unnastrasse 48, 20253 Hamburg, Germany |
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Abstract: | Summary A simple, rapid and reproducible method is presented for the analysis of green-tea extracts in different cosmetic formulations
and in in-vitro skin extracts. Cosmetically active principal components were used for determination of complex assembled green-tea
extracts. The catechins selected were catechin, epicatechin, epigallocatechin, epigallocatechin gallate, and epicatechin gallate,
because of their efficacy and their concentrations in green-tea extracts. The determination was performed by high-performance
liquid chromatography (HPLC), on a reversed-phase (RP) column, coupled to a single-quadrupole mass spectrometer (MS) via an
electrospray ionization (ESI) interface. Detection was performed in the negative selected-ion-monitoring (SIM) mode. A detection
limit between 5 and 15 ng g−1 was achieved in methanol-water-ascorbic acid extracts from different emulsions. A routine analytical procedure could be established
with good quantitative reliability. During validation, the repeatabilities (relative standard deviation) for catechin standard
solutions were found to be 1.1–2.7% (within one day) and 2.2–4.3% (day-to-day). Recoveries from spiked placebos were 98–105%.
The method was successfully used to determine the storage stability of green-tea extract in cosmetic formulations and the
in-vitro penetration of green-tea extract into the skin. |
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Keywords: | Column liquid chromatography Electrospray-ionization mass spectrometry Green-tea catechins Cosmetic formulations In-vitro skin penetration |
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