锌对酒精性肝病小鼠的作用研究

为探究膳食补充锌是否改善肝再生,选用12周大的雄性C57BL/6小鼠40只,按随机数字表法分为4组:正常对照组、酒精中毒组、正常补锌组及酒精中毒补锌组,予不同的饮食喂养6个月后,通过增殖细胞核抗原(PCNA)阳性标记的肝细胞数目评价了肝细胞再生状况,并检测了超氧化物歧化酶(SOD)及丙二醛(MDA)含量评估肝损伤的氧化...

Effects of Zinc Supplementation on Alcoholic Liver Disease Mice

To examine whether dietary zinc supplementation could improve liver regeneration,40 adult C57 BL/6 mice were randomly divided into four groups: control,zinc supplementation,ethanol poisoning and zinc supplementation plus ethanol poisoning.Two groups(ethanol poisoning and zinc supplementation plus ethanol poisoning) were fed an ethanol-containing liquid diet for 6 months developed alcoholic liver disease.Liver regeneration was assessed by immunohistochemical staining of proliferating cell nuclear antigen(PCNA),and as to assess the status of oxidative stress of the mice,the content of MDA and SOD was detected.The results showed that compared with the ethanol poisoning group,the number of proliferation cell nuclear antigen(PCNA)-positive hepatocytes in zinc supplementation plus ethanol poisoning increased significantly.And compared with the control group,the content of MDA increased significantly in the ethanol group,while the content of SOD declined signicantly(P<0.05).In the contrast,the content of SOD increased in the zinc plus ethanol group and MDA decreased significantly compared with the ethanol group(P<0.05).It conclud long term ethanol exposure can lead to high level of peroxide and antioxidation activity fall off,whlie zinc supplementation can reverse the un-balance status.Zinc-enhanced liver regeneration suggesting that dietary zinc supplementation may have beneficial effects in alcoholic liver disease.