|
|||||
|
|
Site-specific conjugation of ScFvs antibodies to nanoparticles by bioorthogonal strain-promoted alkyne-nitrone cycloaddition |
|
| Authors: | Colombo Miriam Sommaruga Silvia Mazzucchelli Serena Polito Laura Verderio Paolo Galeffi Patrizia Corsi Fabio Tortora Paolo Prosperi Davide |
| Institution: | NanoBioLab, Dipartimento di Biotecnologie e Bioscienze, Università di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy. |
| Abstract: | Particularly suitable: An N-terminal serine mutant of anti-HER2 scFv antibody was conjugated to polymer-coated magnetofluorescent nanoparticles by strain-promoted alkyne-nitrone cycloaddition. The resulting nanoparticles (see scheme) proved effective in targeting and labeling HER2-positive breast cancer cells. |
| Keywords: | bioorthogonal reaction cell targeting cycloaddition nanoparticles scFv antibody |
| 本文献已被 PubMed 等数据库收录！ | |