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Freeze-fracture electron microscopic and calorimetric studies on microscopic states of surface-modified liposomes with poly(ethylene glycol) chains |
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| Authors: | Kaname Hashizaki Chika Itoh Hideki Sakai Shoko Yokoyama Hiroyuki Taguchi Yoshihiro Saito Naotake Ogawa Masahiko Abe |
| Affiliation: | a Faculty of Science and Technology, Science University of Tokyo, 2641, Yamazaki, Noda, Chiba 278-8510, Japan b College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba 274-8555, Japan c Oleo Chemicals research laboratory, NOF Co., 3-3, Chidori-cho, Kawasaki-ku, Kawasaki, Kanagawa 210-0865, Japan d Institute of Colloid and Interface Science, Science University of Tokyo, 1-3, Kagurazaka, Shinjuku, Tokyo 162-0825, Japan e Kyoritsu College of Pharmacy, 1-5-30, Shibakoen, Minato-ku, Tokyo 105-8512, Japan |
| Abstract: | The differential scanning calorimetry (DSC) and the freeze-fracture electron microscopy of dipalmitoyl phosphatidylcholine (DPPC) liposomes containing distearoyl-N-monomethoxy poly(ethylene glycol)-succinyl-phosphatidylethanolamines (PEG-DSPE) were carried out. The DSC peak of DPPC liposomes containing PEG-DSPE had a shoulder. The main phase transition temperature of DPPC bilayer membranes containing PEG-DSPE whose molecular weight of PEG is less than 3000 was slightly shifted to a higher temperature, while that containing PEG-DSPE whose molecular weight of PEG is more than 5000 was slightly shifted to a lower temperature. The electron micrographs of freeze-fracture replicas of DPPC liposomes containing PEG-DSPE quenched from 37±2°C exhibited banded and planar textures, suggesting the lateral phase separation in the bilayer membranes. |
| Keywords: | Liposomes Phospholipid with poly (ethylene glycol) chain Freeze-fracture electron microscopy Phase transition temperature Differential scanning calorimetry |
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