Determination of piroxicam and its major metabolites in the plasma, urine and bile of humans by high-performance liquid chromatography. |
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| Authors: | P A Milligan |
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| Affiliation: | University of Glasgow, Department of Medicine and Therapeutics, Western Infirmary, Scotland, UK. |
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| Abstract: | A simple and sensitive liquid chromatographic method with ultraviolet detection is described for the determination of the nonsteroidal anti-inflammatory drug piroxicam and its major metabolites in human plasma, urine and bile. Separation of these components occurs on a reversed phase C10CN column with a mobile phase consisting of acetonitrile-water-sodium dihydrogenphosphate solution. The detection limit of the assay was 50 ng/ml with intra- and inter-assay coefficients of variation for piroxicam of the order of 2 and 5%, respectively. The assay linearity was good (typically r = 0.9999). This method can be readily utilised for clinical pharmacokinetic and mass-balance studies. |
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