| 荧光光谱法研究酸性大红3R与牛血清白蛋白的结合作用 |
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| 引用本文： | 金艳红,李来生,陈会明,何小英. 荧光光谱法研究酸性大红3R与牛血清白蛋白的结合作用[J]. 南昌大学学报(理科版), 2009, 33(4): 1 |
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| 作者姓名： | 金艳红 李来生 陈会明 何小英 |
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| 作者单位： | 南昌大学分析测试中心； |
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| 基金项目： | 江西省教育厅基金资助项目(JJ2007-18) |
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| 摘 要： | 在Tris缓冲溶液(pH 7.4)体系中,运用荧光光谱法和紫外光谱法首次研究了染料酸性大红3R(AS)与牛血清白蛋白(BSA)的相互作用,并考察了表面活性剂对体系荧光强度的影响。结果表明,酸性大红3R与牛血清白蛋白能形成基态复合物,从而导致BSA内源荧光猝灭,猝灭机理主要涉及静态猝灭和非
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| 关 键 词： | 紫外光谱法 荧光光谱法 酸性大红3R 相互作用 牛血清白蛋白 |
Study on the Interaction between Acid Scarlet Red 3R and Serum Albumin Bovine by Fluorescence Spectrometric Method |
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| JIN Yan-hong,LI Lai-sheng,CHEN Hui-ming,HE Xiao-ying. Study on the Interaction between Acid Scarlet Red 3R and Serum Albumin Bovine by Fluorescence Spectrometric Method[J]. Journal of Nanchang University(Natural Science), 2009, 33(4): 1 |
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| Authors: | JIN Yan-hong LI Lai-sheng CHEN Hui-ming HE Xiao-ying |
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| Affiliation: | The Center of Analysis and Testing;Nanchang University;Nanchang 330047;China |
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| Abstract: | The interaction between acid scarlet red 3R(AS) and bovine serum albumin(BSA) in aqueous solution(Tris-HCl buffer,pH 7.4) was studied by fluorescence spectrometric and UV/Vis spectroscopic methods.The effect of surfactants on the interaction between BSA and AS in solution was investigated.The results showed that the fluorescence quenching of BSA by acid scarlet red 3R was a result of the formation of BSA-AS complex,in which both static quenching and non-radioactive energy transferring occurred.According to ... |
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| Keywords: | fluorescence spectroscopy UV/Vis spectroscopy acid scarlet red 3R bovine serum albumin interaction |
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