Substrate specificity of NovM: implications for novobiocin biosynthesis and glycorandomization |
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Authors: | Albermann Christoph Soriano Aileen Jiang Jiqing Vollmer Heidi Biggins John B Barton William A Lesniak Jacob Nikolov Dimitar B Thorson Jon S |
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Affiliation: | Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, Wisconsin 53705, USA. |
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Abstract: | [reaction: see text] In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced. |
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