| 寡糖—8—氨基芘—1,3,6—三磺酸衍生物的毛细管电泳—激光诱？… |
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| 引用本文： | 党福全 陈义. 寡糖—8—氨基芘—1,3,6—三磺酸衍生物的毛细管电泳—激光诱？…[J]. 高等学校化学学报, 2000, 21(2): 206-209 |
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| 作者姓名： | 党福全 陈义 |
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| 作者单位： | 中国科学院化学研究所分子科学中心!北京100080 |
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| 基金项目： | 国家杰出青年基金!(批准号 :29825112)，中国科学院重大基金!(批准号:KJ951-A1-507)资助 |
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| 摘 要： | 利用自组装的毛细管电泳－激光诱导荧光装置，研究了多种寡糖－８－氨基芘－１，３，６－三磺酸（寡糖ＡＰＴＳ）衍生物的分离。考察了电泳介质、浓度及pＨ对寡糖－ＡＰＴＳ 衍生物分离的影响，在酸性和碱性条件下，分别实现了痕量寡糖标准品及葡聚糖水解产物的高效分离。
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| 关 键 词： | 寡糖 葡聚糖 毛细管电泳 激光诱导荧光 分离 |
Ultrasensitive Fluorometric Detection of Oligosaccharides as Their APTS Derivatives by Capillary Electrophoresis |
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| DANG Fu-Quan,CHEN Yi,GUO Qing,XU Gui-Yun. Ultrasensitive Fluorometric Detection of Oligosaccharides as Their APTS Derivatives by Capillary Electrophoresis[J]. Chemical Research In Chinese Universities, 2000, 21(2): 206-209 |
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| Authors: | DANG Fu-Quan CHEN Yi GUO Qing XU Gui-Yun |
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| Abstract: | Reducing oligosaccharides, after reductive amination, were separated and detected as their APTS derivatives by capillary electrophoresis with laser-induced fluorescence. Brief optimization studies have also been performed with respect to CE separation conditions of oligosaccharides. The oligosaccharide ladders were analyzed in an acidic phosphate-tris buffer(pH 25) or in the alkaline borate buffer. Acid hydrolysis products of dextran T-40 labeled with APTS were also successfully mapped. |
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| Keywords: | 8-Aminopyrene-1 3 6-trisufonate(APTS) Oligosaccharide Dextran Capillary electropho-resis with laser-induced fluorescence detection |
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