| 实时生物分子相互作用分析技术用于链霉亲和素-生物素化抗体的层层组装研究 |
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| 引用本文： | 裴仁军,崔小强,杨秀荣,汪尔康.实时生物分子相互作用分析技术用于链霉亲和素-生物素化抗体的层层组装研究[J].高等学校化学学报,2002,23(2):195-198. |
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| 作者姓名： | 裴仁军 崔小强 杨秀荣 汪尔康 |
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| 作者单位： | 中国科学院长春应用化学研究所电分析化学开放研究实验室,长春,130022 |
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| 基金项目： | 国家自然科学基金 (批准号 :2 0 845 0 0 2 )资助 |
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| 摘 要： | 使用生物分子相互作用分析（Biomolecular interaction analysis,BIA)技术实时监测了在链霉素和素表面层层组装亲和素－生物素化抗体多层膜的过程，结果表明，通过链霉素和素与生物素之间的强亲和作用，能够在表面形成均一的多层膜，并用实时BIA技术求得了每层蛋白质的表面浓度，对于生物素化抗体，单层吸附表面浓度为1.32ng/mm^2；对于链霉亲和素，单层吸附表面浓度为2.93ng/mm^2。同时对蛋白质在表面的排列状态进行了探讨。
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| 关 键 词： | 表面等离子体子共振 层层组装 链霉亲和素 生物素化抗体 生物分子相互作用分析技术 亲和作用力 |
| 文章编号： | 0251-0790(2002)02-0195-04 |
Layer-by-Layer Assembly of Multilayer Films Composed of Streptavidin and Biotinylated Antibody by Real-time Biomolecular Interaction Analysis |
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| PEI Ren Jun,CUI Xiao Qiang,YANG Xiu Rong ,WANG Er Kang.Layer-by-Layer Assembly of Multilayer Films Composed of Streptavidin and Biotinylated Antibody by Real-time Biomolecular Interaction Analysis[J].Chemical Research In Chinese Universities,2002,23(2):195-198. |
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| Authors: | PEI Ren Jun CUI Xiao Qiang YANG Xiu Rong WANG Er Kang |
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| Institution: | PEI Ren Jun,CUI Xiao Qiang,YANG Xiu Rong *,WANG Er Kang |
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| Abstract: | Layer by layer assembly of multilayer films of streptavidin and biotinylated antibody was completed on the streptavidin coated surface. Real time biomolecular interaction analysis(BIA) based on surface plasmon resonance technique was used to monitor the multilayer assembly in solution continuously. The results indicate that the uniform multilayer film can be fabricated successfully based on the strong interaction between streptavidin and biotin. The mean surface mass concentration of each adsorption layer is 1 32 ng/mm 2 for biotinylated antibody, 2 93 ng/mm 2 for streptavidin, according to the correlation of SPR response with surface concentration. |
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| Keywords: | Surface plasmon resonance Real time biomolecular interaction analysis(BIA) Layer by layer assembly Streptavidin Biotinylated antibody |
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