Monomolecular films of cholesterol oxidase and S-Layer proteins |
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Authors: | Helen Conceição Ferraz Juliana Aguilar Guimarães Tito Livio Moitinho Alves Carlos José Leopoldo Constantino |
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Institution: | a Chemical Engineering Program, Universidade Federal do Rio de Janeiro, Caixa Postal 68502, Rio de Janeiro, RJ 21941-972, Brazil b Department of Physics, Chemistry and Biology, Universidade Estadual Paulista Júlio de Mesquita Filho, Caixa Postal 467, Presidente Prudente, SP 19060-900, Brazil |
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Abstract: | Cholesterol oxidase (ChOx) is a flavoenzyme that catalyzes the oxidation of cholesterol to cholest-5-en-3-one and subsequently the isomerization to cholest-4-en-3-one. ChOx has been very commonly studied as the detection element in cholesterol biosensors. In the biosensor development field, a relatively new approach is the use of crystalline bacterial cell surface layers, known as S-Layer proteins. These proteins exhibit the ability of self-assembling at surfaces, opening a vast spectrum of applications, both in basic and applied researches. In our study, monomolecular films of ChOx and mixed films of ChOx/S-Layer proteins and DPPC/S-Layer proteins were produced using the Langmuir technique. Characterization of the films was performed by means of surface pressure-molecular area (π-A) isotherms. Stable monolayers were obtained, which means that they can be transferred to solid substrates by Langmuir-Blodgett technique. Mixed monolayers showed an ideal like behavior. |
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Keywords: | Cholesterol Cholesterol oxidase S-Layer proteins Monolayer Biosensor |
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