Effects of O2 and N2/H2 plasma treatments on the neuronal cell growth on single-walled carbon nanotube paper scaffolds |
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| Authors: | Ok Ja Yoon Hyun Jung Lee Yeong Mi JangHyun Woo Kim Won Bok LeeSung Su Kim Nae-Eung Lee |
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| Affiliation: | a School of Advanced Materials Science and Engineering, Center for Advanced Plasma Surface Technology and Center for Human Interface Nanotechnology, Sungkyunkwan University, Suwon, Kyunggi-do 440-746, Republic of Korea
b Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea |
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| Abstract: | The O2 and N2/H2 plasma treatments of single-walled carbon nanotube (SWCNT) papers as scaffolds for enhanced neuronal cell growth were conducted to functionalize their surfaces with different functional groups and to roughen their surfaces. To evaluate the effects of the surface roughness and functionalization modifications of the SWCNT papers, we investigated the neuronal morphology, mitochondrial membrane potential, and acetylcholine/acetylcholinesterase levels of human neuroblastoma during SH-SY5Y cell growth on the treated SWCNT papers. Our results demonstrated that the plasma-chemical functionalization caused changes in the surface charge states with functional groups with negative and positive charges and then the increased surface roughness enhanced neuronal cell adhesion, mitochondrial membrane potential, and the level of neurotransmitter in vitro. The cell adhesion and mitochondrial membrane potential on the negatively charged SWCNT papers were improved more than on the positively charged SWCNT papers. Also, measurements of the neurotransmitter level showed an enhanced acetylcholine level on the negatively charged SWCNT papers compared to the positively charged SWCNT papers. |
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| Keywords: | Single-walled carbon nanotube O2 and N2/H2 plasma treatments Mitochondrial membrane potential Neurotransmitter Scaffolds Neuronal activity |
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