A study of the behaviour of some new column materials in the chromatographic analysis ofCatharanthus alkaloids |
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| Authors: | G Theodoridis I Papadoyannis A Hermans-Lokkerbol R Verpoorte |
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| Institution: | (1) Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University, 540 06 Thessaloniki, Greece;(2) Division of Pharmacognosy, Gorlaeus Laboratories, P.O. Box 9502, 2300 RA Leiden, The Netherlands |
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| Abstract: | Summary New column-packing materials specially designed for the HPLC analysis of basic compounds have been tested for the analysis
ofCatharanthus alkaloids. Mobile phase optimization was performed for each column tested. The influence of mobile phase pH, nature and content
of the organic modifier and salt concentration on retention, selectivity and resolution was studied. An important factor in
the separation proved to be the pH of the eluent, because of the widely different pK
a values of the analytes. Complete separation was easily achieved on ODS columns, but polymeric materials also gave acceptable
results.
Presented at the 21st ISC held in Stuttgart, Germany, 15th–20th September, 1996 |
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| Keywords: | Column liquid chromatography Catharanthus alkaloids New column-packing materials |
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