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DC dielectrophoresis separation of marine algae and particles in a microfluidic chip |
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| Authors: | YongXin Song JianDong Yang XiaoFei Shi Hai Jiang YanBin Wu Ran Peng Qi Wang Ning Gong XinXiang Pan YeQing Sun DongQing Li |
| Affiliation: | 1. Department of Marine Engineering, Dalian Maritime University, Dalian, 116026, China 4. College of Information Science and Technology, Dalian Maritime University, Dalian, 116026, China 3. Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, ON, N2L 3G1, Canada 5. Department of Respiratory Medicine, the Second Hospital Affiliated to Dalian Medical University, Dalian, 116026, China 2. Institute of Environmental Systems Biology, Dalian Maritime University, Dalian, 116026, China |
| Abstract: | This paper reports a microfluidic method of continuous separation of marine algae and particles by DC dielectrophoresis. The locally non-uniform electric field is generated by an insulating PDMS triangle hurdle fabricated within a PDMS microchannel. Both the particles and algae are subject to negative DEP forces at the hurdle where the gradient of local electric-field strength is the strongest. The DEP force acting on the particle or the algae depends on particles’ or algae’s volume, shape and dielectric properties. Thus the moving particles and algae will be repelled to different streamlines when passing the hurdle. In this way, combined with the electroosmotic flow, continuous separation of algae of two different sizes, and continuous separation of polystyrene particles and algae with similar volume but different shape were achieved. This first demonstration of DC DEP separation of polystyrene particles and algae with similar sizes illustrates the great influence of dielectric properties on particle separation and potentials for sample pretreatment. |
| Keywords: | DC dielectrophoresis marine algae particle microfluidic chip permittivity difference |
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