Novel fluorogenic substrates for imaging beta-lactamase gene expression |
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| Authors: | Gao Wenzhong Xing Bengang Tsien Roger Y Rao Jianghong |
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| Institution: | Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, California 90095-1770, USA. |
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| Abstract: | A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after beta-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of beta-lactamase in cell lysates can be readily detected, and beta-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor. |
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