Direct Electrochemistry and Electrocatalysis of Hemoglobin in Lipid Film Incorporated with Room‐Temperature Ionic Liquid |
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| Authors: | Gaiping Li Liangwei Du Hongjun Chen Lixue Zhang Erkang Wang |
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| Affiliation: | 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.?R. China;2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, P.?R. China |
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| Abstract: | A facile phospholipid/room‐temperature ionic liquid (RTIL) composite material based on dimyristoylphosphatidylcholine (DMPC) and 1‐butyl‐3‐methylimidazolium hexafluorophosphate ([bmim]PF6) was exploited as a new matrix for immobilizing protein. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were adopted to characterize this composite film. Hemoglobin (Hb) was chosen as a model protein to investigate the composite system. UV‐vis absorbance spectra showed that Hb still maintained its heme crevice integrity in this composite film. By virtue of the Hb/DMPC/[bmim]PF6 composite film‐modified glassy carbon electrode (GCE), a pair of well‐defined redox peaks of Hb was obtained through the direct electron transfer between protein and underlying GCE. Moreover, the reduction of O2 and H2O2 at the Hb/DMPC/[bmim]PF6 composite film‐modified GCE was dramatically enhanced. |
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| Keywords: | Phospholipid Room‐temperature ionic liquid Hemoglobin Direct electrochemistry Biocatalytic activity |
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