Direct Electrochemistry of Hemoglobin Entrapped in Composite Electrodeposited Chitosan‐Multiwall Carbon Nanotubes and Nanogold Particles Membrane and Its Electrocatalytic Application |
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Authors: | Chuanyin Liu Jiming Hu |
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Institution: | 1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P.?R. China;2. Department of Chemistry, Yunyang Teachers College, Danjiangkou 442700, P.?R. China |
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Abstract: | Hemoglobin was entrapped in composite electrodeposited chitosan‐multiwall carbon nanotubes (MCNTs) film by assembling gold nanoparticles and hemoglobin step by step. In phosphate buffer solution (pH 7), a pair of well‐defined and quasireversible redox peaks appeared with formal potential at ?0.289 V and peak separation of 100 mV. The redox peaks respected for the direct electrochemistry of hemoglobin at the surface of chitosan‐MCNTs‐gold nanoparticles modified electrode. The parameters of experiments have also been optimized. The composite electrode showed excellent electrocatalysis to peroxide hydrogen and oxygen, the peak current was linearly proportional to H2O2 concentration in the range from 1×10?6 mol/L to 4.7×10?4 mol/L with a detection limit of 5.0×10?7 mol/L, and this biosensor exhibited high stability, good reproducibility and better selectivity. The biosensor showed a Michaelis–Menten kinetic response as H2O2 concentration is larger than 5.0×10?4 mol/L, the apparent Michaelis–Menten constant for hydrogen peroxide was calculated to be 1.61 μmol/L. |
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Keywords: | Hemoglobin Direct electrochemistry Electrocatalysis Electrodeposited chitosan‐MCNTs membrane |
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