A Mediator‐Free Bienzyme Amperometric Biosensor Based on Horseradish Peroxidase and Glucose Oxidase Immobilized on Carbon Nanotube Modified Electrode |
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| Authors: | Yan‐Li Yao Kwok‐Keung Shiu |
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| Institution: | Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong |
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| Abstract: | Multiwalled carbon nanotube (CNT) modified glassy carbon electrode immobilized with horseradish peroxidase (HRP) in Nafion coating showed direct electron transfer between HRP enzyme and the CNT‐modified electrode. A mediator‐free bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase was constructed. The bienzyme biosensor exhibited a high sensitivity for glucose detection at zero applied potential. |
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| Keywords: | Bienzyme glucose biosensor Glucose oxidase Horseradish peroxidase Direct electrochemistry Carbon nanotubes Mediator‐free |
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