Dual‐Genic Hybridization Sensor Employing Electrochemical Impedance Spectroscopy |
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| Authors: | Alessandra Bonanni Daniel Calvo Manuel del Valle |
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| Affiliation: | Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, Edifici Cn, 08193?Bellaterra, Spain |
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| Abstract: | A dual‐genic impedimetric sensor for DNA hybridization detection is proposed for the first time. Two different single‐stranded DNA probes (P1=d(pA)20 and P2=d(pG)20) were immobilized onto the graphite‐epoxy electrode surface and impedimetric measurements were performed. Hybridization experiments were carried out employing complementary targets (P1c=d(pT)20 or P2c=d(pC)20) alone, or combined (P1c+P2c), as well as noncomplementary DNA sequences. As from the direct observation of impedance spectra it was not possible to resolve the binary gene mixture, the use of artificial neural networks (ANN) was proposed for extracting significant information to get desired results. |
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| Keywords: | Impedance sensors DNA Dual‐genic detection Artificial neural networks |
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