High Sensitive Electrochemical Detection of Sequence‐Specific DNA Using Low Current Voltammetry |
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| Authors: | Lingling Lin Jianzhang Zhou Yan Zhang Zhonghua Lin |
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| Affiliation: | State Key Laboratory of Physical Chemistry of the Solid Surface and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, P.?R.China |
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| Abstract: | In this article, we report on efforts to construct a high sensitive electrochemical sensor with immobilized sandwich‐type DNA borne ferrocene (Fc) head for sequence‐specific DNA detection using ultramicroelectrode and low current voltammetry. Based on the difference in deformability between the bending rigid complementary DNA double helix and its anomalous flexile mismatches, the fully complementary target can be distinguished from mismatched targets including the single‐base mismatched target. Detection limit estimated as the amount of DNA is observed to be 100 fM via low current voltammetry. The method offers great promise of high sensitivity and selectivity simultaneously for effective gene identification. |
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| Keywords: | Electrochemical detection Sandwich‐type DNA Mismatch False positive hybridization Deformability |
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